RNA

Part:BBa_K3841087:Design

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-10-18)


gRNA for CRISPR neutral insertion site 4 in Komagataella phaffii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The crRNA is complementary to chromosome 4 (position 1633755-1633774) within the assembled genome of K. phaffii GS115 strain by De Schutter et al. 2009 (Accession number FN392322).

References